Profile
Educational background 
Ph.D (2003) Southwest Agricultural University
Bsc (1997)   Southwest Agricultural University

Positions held
2012   -Full professor Zhejiang University
2012   - Adjunct professor University of Maryland College Park

2003-2004 -Assistant (2003) and associate (2004) professor Southwest Agricultural University
2004-2006 -Post-Doc Brock University ON Canada

2007-2012  -Research Associate Department of Entomology University of Maryland College Park MD USA
Research interests: 

Evolution and genetic of host pathogen interactions; entomopathogenic fungi; symbionts and plant interactions; functional genomics; molecular biology of fungi; signal transduction pathways; secondary metabolites; biotechnology; vector-borne disease control; insect pest control; detoxification of heavy metals.

Publications 
    1. Xu C, Zhang X, Qian Y, Chen X, Liu R, Zeng G, Zhao H, Fang W*. A high-throughput gene disruption methodology for the entomopathogenic fungus Metarhizium robertsii. PLoS ONE (accepted)
    2. Liao X*, O'Brien TR, Fang W, St Leger RJ.  The plant beneficial effects of Metarhizium species correlate with their association with roots. Appl Microbiol Biotechnol. 2014 May 8. [Epub ahead of print]
    3. Zhao H, Xu C, Lu HL,  Chen X, St.Leger RJS, Fang W* Host-to-pathogen gene transfer facilitated infection of insects by a pathogenic fungus. PLoS Pathogens  2014 Apr 10;10(4):e1004009. doi: 10.1371/journal.ppat.1004009.  *:corresponding author   Digested by Microbe World: http://www.microbeworld.org/podcasts/this-week-in-microbiology/archives/1677-twim-78-a-bacterium-grows-in-brooklyn
    4. Shi W*, Guo Y, Xu C, Tan S, Miao J,Feng Y, Zhao H, St. Leger RJ, Fang W* Unveiling the mechanism for preventing migratory locust’s swarm behavior by microsporidian parasites Proc Natl Acad Sci USA 2014 Jan 28;111(4):1343-8 *: corresponding author Highlighted by Science: http://news.sciencemag.org/plants-animals/2014/01/gut-parasite-may-keep-locusts-swarming
    5. Liao X, LU HL, Fang W, St.Leger RJ. Overexpression of a Metarhizium robertsii HSP25 gene increases thermotolerance and survival in soil. Appl Microbiol Biotechnol. 2014 Jan;98(2):777-83.
    6. Liao X, Fang W, Lin L, Lu HL, St.leger RJ. Metarhizium robertsii Produces an Extracellular Invertase (MrINV) That Plays a Pivotal Role in Rhizospheric Interactions and Root Colonization.  PLoS One. 2013 Oct 21;8(10):e78118. doi: 10.1371/journal.pone.0078118.
    7.  Fang W, St. Leger RJ. Enhanced UV Resistance and Improved Killing of Malaria Mosquitoes by Photolyase Transgenic Entomopathogenic Fungi. PLoS One 2012;7(8):e43069
    8. Fang W, Azimzadeh P, St Leger RJ. Strain improvement of fungal insecticides for controlling insect pests and vector-borne diseases. Curr Opin Microbiol. 2012;15:232-238.
    9. Lin L, Fang W, Liao X, Wang F, Wei D, St Leger RJ. The MrCYP52 cytochrome P450monoxygenase gene of Metarhizium robertsii is important for utilizing insect epicuticular hydrocarbons. PLoS One. 2011;6(12):e28984.
    10. Wang S, Fang W, Wang C, St. Leger RJ. Insertion of an esterase gene into a specific locust pathogen (Metarhizium acridum) enables it to infect caterpillars. PLoS Pathog. 2011 7(6):e1002097.
    11. Fang W, Vega-Rodríguez J, Ghosh AK, Jacobs-Lorena M, Kang A, St Leger RJ. Development of transgenic fungi that kill human malaria parasites in mosquitoes. Science. 2011 331(6020):1074-7.
    12. Gao Q, Jin K, Ying SH, Zhang Y, Xiao G, Shang Y, Duan Z, Hu X, Xie XQ, Zhou G, Peng G, Luo Z, Huang W, Wang B, Fang W, Wang S, Zhong Y, Ma LJ, St Leger RJ, Zhao GP, Pei Y, Feng MG, Xia Y, Wang C. Genome sequencing and comparative transcriptomics of the model entomopathogenic fungi Metarhizium anisopliae and M. acridum. PLoS Genet. 2011 6;7(1):e1001264.
    13. Pava-Ripoll M, Angelini C, Fang W, Wang S, Posada FJ, St Leger R. The rhizosphere-competent entomopathogen Metarhizium anisopliae expresses a specific subset of genes in plant root exudate. Microbiology. 2011 157:47-55.
    14. Fang W, St Leger RJ. Mrt, a gene unique to fungi, encodes an oligosaccharide transporter and facilitates rhizosphere competency in Metarhizium robertsii. Plant Physiol. 2010 154(3):1549-57.
    15. Fang W, Fernandes EK, Roberts DW, Bidochka MJ, St Leger RJ. A laccase exclusively expressed by Metarhizium anisopliae during isotropic growth is involved in pigmentation, tolerance to abiotic stresses and virulence. Fungal Genet Biol. 2010 47(7):602-7.
    16. Fang W, St Leger RJ. RNA binding proteins mediate the ability of a fungus to adapt to the cold. Environ Microbiol. 2010 12(3):810-20.
    17. Jin K, Zhang Y, Fang W, Luo Z, Zhou Y, Pei Y. Carboxylate transporter gene JEN1 from the entomopathogenic fungus Beauveria bassiana is involved in conidiation and virulence. Appl Environ Microbiol. 2010 76(1):254-63.
    18. Fang W, Feng J, Fan Y, Zhang Y, Bidochka MJ, Leger RJ, Pei Y. Expressing a fusion protein with protease and chitinase activities increases the virulence of the insect pathogen Beauveria bassiana. J Invertebr Pathol. 2009 102(2):155-9.
    19. Wang S, Leclerque A, Pava-Ripoll M, Fang W, St Leger RJ. Comparative genomics using microarrays reveals divergence and loss of virulence-associated genes in host-specific strains of the insect pathogen Metarhizium anisopliae. Eukaryot Cell. 2009 8(6):888-98.
    20. Zhang Y, Zhao J, Fang W, Zhang J, Luo Z, Zhang M, Fan Y, Pei Y. Mitogen-activated protein kinase hog1 in the entomopathogenic fungus Beauveria bassiana regulates environmental stress responses and virulence to insects. Appl Environ Microbiol. 2009 75(11):3787-95.
    21. Fang W, Pava-ripoll M, Wang S, St Leger R. Protein kinase A regulates production of virulence determinants by the entomopathogenic fungus, Metarhizium anisopliae. Fungal Genet Biol. 2009 46(3):277-85.
    22. Fang W, Scully LR, Zhang L, Pei Y, Bidochka MJ. Implication of a regulator of G protein signalling (BbRGS1) in conidiation and conidial thermotolerance of the insect pathogenic fungus Beauveria bassiana. FEMS Microbiol Lett. 2008 279(2):146-56.
    23. Liao XG, Fang WG, Zhang YJ, Fan YH, Wu XW, Zhou Q, Pei Y. Characterization of a highly active promoter, PBbgpd, in Beauveria bassiana. Curr Microbiol. 2008 57 :121-6.
    24. Fan Y, Fang W, Xiao Y, Yang X, Zhang Y, Bidochka MJ, Pei Y. Directed evolution for increased chitinase activity. Appl Microbiol Biotechnol. 2007 76(1):135-9.
    25. Fang W, Pei Y, Bidochka MJ. A regulator of a G protein signalling (RGS) gene, cag8, from the insect-pathogenic fungus Metarhizium anisopliae is involved in conidiation, virulence and hydrophobin synthesis. Microbiology. 2007 153:1017-25.
    26. Fan Y*, Fang W*, Guo S, Pei X, Zhang Y, Xiao Y, Li D, Jin K, Bidochka MJ, Pei Y. Increased insect virulence in Beauveria bassiana strains overexpressing an engineered chitinase. Appl Environ Microbiol. 2007 73(1):295-302. (*: contribute equally)
    27. Fang W, Bidochka MJ. Expression of genes involved in germination, conidiogenesis and pathogenesis in Metarhizium anisopliae using quantitative real-time RT-PCR. Mycol Res. 2006 110:1165-71.
    28. Fang W, Pei Y, Bidochka MJ. Transformation of Metarhizium anisopliae mediated by Agrobacterium tumefaciens. Can J Microbiol. 2006 52(7):623-6
    29. Yang X, Li J, Wang X, Fang W, Bidochka MJ, She R, Xiao Y, Pei Y. Psc-AFP, an antifungal protein with trypsin inhibitor activity from Psoralea corylifolia seeds. Peptides. 2006 27(7):1726-31.
    30. Fang W, Leng B, Xiao Y, Jin K, Ma J, Fan Y, Feng J, Yang X, Zhang Y, Pei Y. Cloning of Beauveria bassiana chitinase gene Bbchit1 and its application to improve fungal strain virulence. Appl Environ Microbiol. 2005 71(1):363-70.
    31. Fang W, Zhang Y, Yang X, Zheng X, Duan H, Li Y, Pei Y. Agrobacterium tumefaciens-mediated transformation of Beauveria bassiana using an herbicide resistance gene as a selection marker. J Invertebr Pathol. 2004 Jan;85(1):18-24.
    32. Fang W, Zhang Y et al. Cloning and Characterization of cuticle degrading enzyme CDEP-1. Yi Chuan Xue Bao 2002 29:278-282
    33. Fang W, Pei Y et al. Rapid extraction of fungal genomic DNA. Chinese Journal of Applied & Environmental Biology 2002 8:305-307
    34. Fang W, Zhang Y et al. Fungal transformation mediated by Agrobacterium tumefaciens, a review. China Biotechnology 2002 22:41-45.
    35.  Fang W, Zhang Y et al. Isolation and characterization of a carboxylic transport protein JEN1 and its promoter from Metarhizium anisopliae. Yi Chuan Xue Bao 2003 30:283-288.
    36. Fang W, Zhang Y et al. Cloning and analysis of the promoter of subtilisin-like protease gene cdep-1 from Beauveria bassiana by YADE method. Mycosystema 2003 22:252-258.
    37. Fang W, Wei Y et al. An Effective Silver Staining Protocol for DNA. HEREDITAS(BEIJING) 2000 22:167-168.
    38. Fang W, Zhang Z et al. Cotton Marker for Boll Weight Based on AFLP. ACTA GOSSYPII SINIA 2000 12:176-179.
    39. Zhang Y, Fang W et al. Cloning and Characterization of FUS3/KSS1-like protein BbMPK1 Mycosystema 2005 24:104-111.
    40. Luo Z, Fang W et al. Optimization of the Fermentation Conditions to Increase the Production of the Subtilisin-like Protease in a Recombinant Strain of Beauveria bassiana. Journal of Agricultural Biotechnology 2005 13:372-376
    41. 34. Fan Y, Fang W et al. Preparation of a serine protease CDEP-1 of Beauveria bassiana by Pichia pastoris expression system. Mycosystema 2006 25:56-62.
    42. Zhou Z, Fang W et al. The cloning and sequence analysis of cDNA fragment of soybean ferritin gene and construction of its expression vector for plant transformation. Southwest China Journal of Agricultural Sciences 2002 15.
    43.  Pei Y, Fang W et al. Mechanism of Fungal Pathogenesis in Insect and Strain Improvement by Gene Engineering. Journal of Agricultural Biotechnology 2003 11:221-226.
    44. Zhang Y, Fang W et al. Cloning and Characterization of a Carboxylic Transport Protein JEN1 and Its Upstream Sequence from Beauveria bassiana. Acta Microbiologica Sinica. 2004 44:198-201.
    45. Li D, Fang W et al. Amplification of Pollen Fertility Gene MS2Bnap Full-length cDNA from Brassica napus Using SOE by PCR. Hereditas (BEIJING) 2001 23:326-331
    46. 39. Xiao Y, Fang W et al. PCR walking in cotton genome using YADE method. Yi Chuan Xue Bao. 2002 29:62-66.
    47. Xiao Y, Fang W et al. Rapid Amplification of Cotton Ovule cDNA Ends by Y-RACE. Chinese Journal of Biochemistry and Molecular Biology 2002 18:688-692.
    48. Zhang Y, Fang W et al. Synergistic effect of fermented solution produced by transgenic Beauveria bassiana increasing the insecticidal efficacy of wild-type strain results from subtilisin-like activity. Journal of Plant Protection 2006 33:246-250.
    49.  Zhang Y, Fang W et al. Screen of isolates of entomopathogenic fungi with relatively high tolerance to desiccation and virulence. Mycosystema 2001 20:73-78.
    50. Zhang Y, Fang W et al. Induction of extracellular protease and subtilisin-like protease of Beauveria bassiana. Chinese Journal of Applied & Environmental Biology 2000 6: 182-186

Patents

1. Weiguo Fang & Raymond J St. Leger. Novel Genetic Containment Strategy for Biological Pest Control Agents in Soil (LS-2011-105). 2. Weiguo Fang & Raymond J St. Leger. Novel Environmentally Safe Anti-Malarial and Dual Bio-Containment Strategies for Biological Control Agents ( LS-2011-106). 3. Pei Y, Fan Y, Zhang Y, Fang W and Xiao Y. Mycoinsecticide supplemented with recombinant protease(Patent no. CN1763187) 4. Zhang Y, Pei Y, Yang X and Fang W. (Patent no. CN1329829)

 

 

Contact

Phone:
E-mail:wfang1@zju.edu.cn