Research Interests:
Functional genomics, Crop improvement through genetic engineering, Plant transformation technology development
Current Appointment
2006-present, Head, Department of Biotechnology
2006-present, Associate director, Plant Science Institute
2004 -present, Professor, College of Life Sciences, Zhejiang University, China
2013-present, "Qiushi" professor,  Zhejiang University, China
Ph. D (Plant Breeding), January 1999 – June 2003, Plant Transformation Facility, Department of Agronomy, Iowa State University, USA.
Dissertation title: Crop improvement through genetic engineering: Development of transformation technologies and production of stress tolerant transgenic crops.
M.S. (Plant Genetics and Breeding), September 1985 – July 1988, Department of Agronomy, Zhejiang Agricultural University (Renamed as Zhejiang University), China.
        Dissertation title: Genetic and clustering analyses on spring and summer soybean germplasm from Zhejiang province. 
B.S. (Seed Science and Technology), September 1981 – July 1985, Department of Agronomy, Zhejiang Agricultural University (Renamed as Zhejiang University), China.
Teaching and Research Experiences
January, 2004 – Present, Professor, Zhejiang University, China.
1) Teaching the graduate course “Genomics” and undergraduate course “Molecular Biology”.
2) Leading a rice functional genomics lab to isolate genes involving in phosphate uptake efficiency, iron metabolism, iron bioavailability, plant root development and transformation efficiency.
3) Administration responsibilities as the head of Department of Biotechnology and associate director of Plant Science Institute
July, 2006 – Sept, 2006, Consultant, Plant Breeding, Gene & Biotech Division, IRRI
June, 2003 – January, 2004, Postdoctoral Research Associate, Plant Transformation Facility, Department of Agronomy, Iowa State University, USA
January 1999 – June, 2003, Graduate Research Associate, Plant Transformation Facility, Iowa State University. 
August 1998 – January, 1999, Visiting Scientist, Plant Transformation Facility, Department of Agronomy, Iowa State University, USA
August 1994 – August 1998, Lecturer, Seed Science Center, Zhejiang Agricultural University, China
August 1988 – August 1994, Research Scientist, Soybean Genetics, Breeding and Extension at Crop Institute, Zhejiang Academy of Agricultural Sciences, China
Honors and Awards
2013, Qiushi Professorship, Zhejiang University.
2009, Outstanding young scientist in Zhejiang Province, China
2007. My teaching course “Molecular Biology” was nominated as one of the Nation’s 100 Bilingual Teaching Demonstration Courses, recognized by the Ministry of Education, China.
2005. New Century Excellent Young Scholars, recognized by the Ministry of Education, China.
2005. Teaching Excellent Award, recognized by Zhejiang University, China
2005. Employee Excellence Award, recognized by the College of Life Sciences, Zhejiang University, China.
2003. Research Excellence Award, recognized by the Graduate College, Iowa State University, USA
Recent Scientific Publications

 Wang C, Yue WH, Ying YH, Wang SD, Secco D, Liu Y, Whelan J, Tyerman S, Shou HX*. 2015. Rice SPX-Major Facility Superfamily3, a vacuolar phosphate efflux transporter, is involved in maintaining phosphate homeostasis in rice. Plant Physiol. 169: 2822-2831.

         Secco D, Wang C, Shou HX, Schultz MDChiarenza S,  Nussaume L, Ecker J, Whelan J, Lister R. 2015.  Stress induced gene expression drives transient DNA methylation changes at adjacent repetitive elements. eLife 2015;4:e09343.
         Zhou L, Wang C, Liu RF, Han Q, Rebecca VK, Juan D, Tyerman S, Shou HX*. 2014. Constitutive overexpression of soybean plasma membrane intrinsic protein GmPIP1;6 confers salt tolerance. BMC Plant Biol. 14:181  doi:10.1186/1471-2229-14-181
         Zhou L, He HL, Liu RF, Han Q, Liu B, Shou HX*.  2014. Overexpression of GmAKT2 potassium channel enhances resistance to soybean mosaic virus. BMC Plant Biol14:154 doi:10.1186/1471-2229-14-154
         Wang C, Li S, Ng S, Zhang BC, Zhou YH, Whelan J, Wu P,  Shou HX. 2014. Mutation in xyloglucan 6-xylosytransferase results in abnormal root hair development in Oryza sativa. J. Exp. Bot.  doi:10.1093/jxb/eru189
         Li S, Wang C, Zhou L, Shou HX, 2014. Oxygen deficit alleviates phosphate overaccumulation toxicity in OsPHR2 overexpression plants. J Plant Res 127:433-440.
         Narsai R, Devenish J, Castleden I, Narsai K, Xu L, Shou HX, Whelan J. 2013. Rice DB: an Oryza Information Portal linking annotation, subcellular location, function, expression, regulation, and evolutionary information for rice and Arabidopsis. Plant J 76: 1057–1073
         Secco D, Shou HX, Whelan J, Berkowitz O. 2014. RNA-seq analysis identifies an intricate regulatory network controlling cluster root development in white lupin. BMC Genom. 15:230 doi:10.1186/1471-2164-15-230
     Song ZY, Tian JL, Fu WZ, Li L, Lu LH, Zhou L, Shan ZH, Tang GX, Shou HX*. 2013. Screening Chinese soybean genotypes for Agrobacterium-mediated genetic transformation suitability. J Zhejiang Univ Sci B. 14:289-198
     Wu P, Shou HX, Xu GH, Lian XM, 2013. Improvement of phosphorus efficiency in rice on the basis of understanding phosphate signaling and homeostasis. Curr Opin Plant Biol. 16: 205212
     Secco D, Jabnoune M, Walker H, Shou HX, Wu P, Poirier Y, Whelan J, 2013. Spatio-Temporal Transcript Profiling of Rice Roots and Shoots in Response to Phosphate Starvation and Recovery. Plant Cell. 25:4285-4304.
     Wang L, Ying YH, Narsai R, Ye L, Zheng L, Tian J, Whelan J, Shou HX*. 2013. Identification of OsbHLH133 as a regulator of iron distribution between roots and shoots in Oryza sativa. Plant Cell & Envir. doi: 36:224-236
     Wang C, Huang W, Ying YH, Li S, Secco D, Tyerman S, Whelan J, Shou HX*. 2012. Functional characterization of the rice SPX-MFS family reveals a key role of OsSPX-MFS1 in controlling phosphate homeostasis in leaves. New Phytol. 196 139–148
     Tian JL, Wang C, Zhang Q, He XW, Whelan J, Shou HX*. 2012. Overexpression of OsPAP10a, a root-associated acid phosphatase, increased extracellular organic phosphorus utilization in rice. J. Integr. Plant Biol. 54 (9): 631–639
     Secco D, Wang C, Arpat BA, Wang ZY, Poirier Y, Tyerman SD, Wu P, Shou HX, Whelan J. 2012. The emerging importance of the SPX domain-containing proteins in phosphate homeostasis. New Phyt. 193: 842–851
     Secco D, Wang C, Shou HX, Whelan J. 2012. Phosphate homeostasis in the yeast Saccharomyces cerevisiae, the key role of the SPX domain-containing proteins. FEBS Letters 586: 289-295. (IF: 3.5)
   Wu JJ, Wang C, Zheng LQ, Wang L,  ChenYR, Whelan J, Shou HX* . 2011.Ethylene is involved in the regulation of iron homeostasis in rice by regulating the expression of iron-acquisition-related genes in Oryza sativa. J. Exp. Bot. 62667-674.19.     Zheng LQ, Ying YH, Wang L, Wang F, WhelanJ, Shou HX* . 2010. Identification of a novel iron regulated basic helix-loop-helix protein involved in Fe homeostasis in Oryza sativa. BMC Plant Biol. 10166.
     Zheng LQ, Cheng ZQ, Ai CX, Jiang XH, Bei XS, Zheng Y, Jiang XH, Glahn RP, Welch RM, Miller DD, Lei XG, Shou HX*. 2010. Nicotianamine, a novel enhancer of iron bioavailability to humans in rice grain. PLoS One. 2010; 5: e10190. 
     Zhang Q, Wang C, Tian JL, Li K, Shou HX*, 2010. Identification of rice purple acid phosphatases related to Pi-starvation signaling. Plant Biol. 137-15
      Shou HX23.     Li K, Wang C, Ying S, Shou HX*. 2010. Comparing genetic characteristaics of retrotransposon TOS17 during different tissue culture processes in rice cultivars Nipponbare and Shishoubaimao.  Agricultural Sciences in China. 9(2): 157-162.
     Wang C, Ying S, Huang HJ, Li K, Wu P, Shou HX*. 2009. Involvement of OsSPX1 in phosphate homeostasis in rice. Plant J 57:895-904
     Zheng LQ, Huang FL, Narsai R, He F, Giraud E, Wu JJ, Cheng LJ, Wang F, Wu P, Whelan J, Shou HX*. (2009) Physiological and transcriptome analysis of iron and phosphorus interaction in rice seedlings. Plant Physiol. 151: 262-274
     Zheng Y, He XW, Lu JF, Ying YH, Gelvin S, Shou HX*. 2009. Expression of the Arabidopsis HTA1 gene enhances rice transformation efficiency. Mol Plant. 2:832-837
     Liu SP, Wang JR, Wang L, Wang XF, Xue YH, Wu P and Shou HX*, 2009. Adventitious root formation in rice requires OsGNOM1 and is mediated by the OsPINs family. Cell Res. 19:1110–1119
     Wang C, Zhang Q and Shou HX*. 2009. Identification and expression analysis of OsHsfs in rice. J Zhejiang Univ Sci B 10(4):291-300 291
     Cheng LJ*, Wang F*, Shou HX*, Zhao FJ, Zheng LQ, He F, Li JH, Zhao FJ, Ueno D, Ma J-F, & Wu P. (2007). Mutation in nicotianamide aminotransferease stimulated Fe(II) acquisition system and led to iron accumulation in rice. Plant Physiology145: 1647-1657 (*, Co-first author)
     Li JY, He XW, Xu L, Zhou J, Wu P, Shou HX*. 2007. Molecular and functional comparisons of the vacuolar Na+/H+ exchangers originated from glycophytic and halophytic species. J Zhejiang Univ Science B 9132-140* Corresponding author.
     Xu M, Zhu L, Shou HX*, Wu P* 2005. A PIN1 Family Gene, OsPIN1, involved in Auxin-Dependent Adventitious Root Emergence and Tillering in Rice. Plant Cell & Physiol. 46:1674-1681 (*Corresponding author)
     Mao CZ, Ding WN, Wu YR, Yu J, He XW, Shou HX, Wu P. 2007. Overexpression of a NAC-domain protein promotes shoot branching in rice. New Phyt. 176: 288-298
33.     Li X, Mo XL, Shou HX, Wu P. 2006.Cytokinin-Mediated Cell Cycling Arrest of Pericycle Founder Cells in Lateral Root Initiation of Arabidopsis. Plant Cell & Physiol. 47(8):1112-1123
34.     Liu H, Wang S, Yu X, Yu J, He X, Zhang S, Shou HX & Wu P (2005) ARL1, a LOB-domain protein required for adventitious root formation in rice. Plant J. 43:47-56.
35.     Lin T, He XW, Yang Y, Shou HX, Wu P (2005). Identification and characterization of a novel water-stress-suppressed gene OsARD encoding an aci-reductone-dioxygenase-like protein in rice. Gene, 360 : 27-34
     Qi XP, Zhou J, Jia QJ, Shou HX, Chen HM, Wu P. (2005) A characterization of the response to auxin of the small GTPase, Rha1. Plant Sci. 169: 1136-1145
37.     Shou H, Bordallo P, Fan J, Yeakley JM, Bibikova M, Sheen J, Wang K. 2004. Expression of a tobacco MAP kinase kinase kinase enhances freezing tolerance in transgenic maize. Proc Natl Acad Sci 113298-3303
38.     Shou H, Frame B, Witham S, Wang K. 2004. Assessment of transgenic maize events produced by particle bombardment or Agrobacterium-mediated transformation. Mol Breeding 13:201-208
39.     Shou, H, Bordallo P, Sheen J, Wang K. Expression of the Nicotiana protein kinase 1 (NPK1) enhances drought tolerance in transgenic maize. J Exp Bot 55:1013-1019
40.     Shou H, Palmer RG, Wang K. 2002. Irreproducibility of the soybean pollen-tube pathway transformation procedure. Plant Molecular Biology Reporter 20:325-334
Current Funded Research Projects


Ye LX, Li L, Wang L,  Wang SD, Li S, Du J, Zhang SQ, Shou HX*. 2015. MPK3/MPK6 are involved in iron deficiency-induced ethylene production in Arabidopsis. Front. Plant Sci.,
2016-2020, Soybean research, 4,000,000 Yuan , Funded by the National Bureau of Science and Technology. PI
2016-2019, Molecular regulation of OsPHO2 by thrioredoxins and its function on  phosphate homeostasis. 840,000 Yuan , Funded by the National Natural Science Foundation of China. PI
2011-2015,Molecular mechanism of iron deficiency induced ethylene production. 600,000 Yuan , Funded by the National Natural Science Foundation of China. PI
2009-2011, Molecular mechanism of cross-talk between phosphorus and iron signaling pathways in rice. 330,000 Yuan ($47,000), Funded by the National Natural Science Foundation of China. PI
2008-2011, Isolation and functional analysis on four novel iron-responsive transcription factors. 300,000 Yuan ($42,800), Funded by the National Natural Science Foundation of China. PI
2008-2011, Identifying novel gene resource by transforming large fragments DNA from bamboo into rice. 400,000 Yuan ($57,000), Funded by the National Bureau of Science and Technology. PI
2006-2010, Improvement of phosphate using efficiency in rice through genetic engineering. 1,250,000 Yuan ($178,000). Funded by the National Bureau of Science and Technology. PI
2005-2008, Effect of overexpressing phytase gene on the phosphate use efficiency in soybean and rice. 800,000 Yuan ($114,000), Funded by the Provincial Bureau of Science and Technology, and the Ministry of Education. PI
2004-2007, Identification of genes involving in the H2O2 signal pathway in rice and their application in crop improvement. 230,000 Yuan ($32,800), Funded by the National Natural Science Foundation of China. Finished. PI
2005-2007, Development of rice breeding lines with low phytic acid and enhanced iron content. $50,000, Funded by HarvestPlus. Co-P


Research work

Plant functional genomics, Crop improvement through genetic engineering, Plant transformation technology development