Positions and Employment

8/1999-5/2005 Ph.D. thesis research in biophysics with Prof. Tim Baker, Department of Biology Sciences, Purdue University, Lafayette, IN

5/2005-10/2007 Postdoctoral fellow with Prof. Niko Grigorieff, HHMI, Brandeis University, Waltham, MA

10/2007-12/2010 UCLA, Postdoctoral fellow with Prof. Hong Zhou, Dept. of MIMG, UCLA

1/2011 – 2/2016 UCLA, Technical director of the Electron Imaging Center for Nanomachines, California NanoSystems Institute, UCLA

3/2016 – present Professor, Director of the Center of Cryo Electron Microscopy, Zhejiang University School of Medicine

Honors

3/2010  MIMG Departmental nominee for the chancellor’s award for postdoctoral research, UCLA, USA

6/2010  Invited speaker of Gordon Research Conference on 3DEM, Italy

8/2010  Invited speaker of Third K.H. Kuo Summer School of Electron Microscopy and Crystallography, China

9/2011  FineGold award for postdoctoral research, UCLA

8/2012  Invited speaker in Microscopy and Microanalysis 2012, Phoenix, Arizona

2/2013  Invited speaker at the Clinical and Translational Science Lecture, UCLA

7/2014  Selected speaker in Seventh K.H. Kuo Summer School of Electron Microscopy and Crystallography

Publications

1. Zhang, X., Lai, M., Chang, W., Yu, I., Ding, K., Mrazek, J., Ng, H.L., Yang, O.O., Maslov, D.A., Zhou, Z.H. (2016). Structures and stabilization of kinetoplastid-specific split rRNAs revealed by comparing leishmanial and human ribosomes. Nature Communications 7:13223

2. Zhang, X.; Patel, A.; Celma, C.; Yu, X.; Roy, P.; Zhou, H. (2016). Atomic model of a nonenveloped virus reveals pH sensors for a coordinated process of cell entry. Nature Structural & molecular Biology 23 (1), 74-80.

3. Zhang, X., Ke, D.; Yu, X.; Chang, W.; Sun, J.; Zhou, H. (2015). In situ structures of the segmented genome and RNA polymerase complex inside a dsRNA virus. Nature 527 (7579), 531–534. (F1000 recommended)
   

4. Zhang, X.; Jin, L.; Fang, Q.; Hui, W.; Zhou, Z.H. (2010). 3.3 Å Cryo-EM Structure of a Nonenveloped Virus Reveals a Priming Mechanism for Cell Entry, Cell 141(3):472-482. PMCID: PMC3422562. (cover paper and F1000 recommended)

5. Zhang, X. Guo, H., Jin L., Czornyj E., Hodes, A., Hui, W.H., Nieh, A.W., Miller, J.F. and Zhou, Z.H. (2013) A new topology of the HK97-like fold revealed in Bordetella bacteriophage by cryoEM at 3.5Å resolution. eLife, 2:e01299.

6. Zhang, X. and Zhou, Z.H. (2011) Limiting factors in atomic resolution cryo electron microscopy – No simple tricks. J. Struct. Biol., 175, 253-263.
   

7. Zhang, X.; Boyce, M.; Bhattacharya, B.; Zhang, X.; Schein, S.; Roy, P.; Zhou, Z.H. (2010). Bluetongue virus coat protein VP2 contains sialic acid-binding domains, and VP5 resembles enveloped virus fusion proteins, PNAS 107(14):6292-6297. PMCID: PMC2852009.

8. Zhang, X., E. Settembre, C. Xu, P. R. Dormitzer, R. Bellamy, S. C. Harrison and N. Grigorieff (2008). "Near-atomic resolution using electron cryomicroscopy and single-particle reconstruction." PNAS 105(6): 1867-1872.

9. Zhang, X.; Ji, Y.; Zhang, L.; Harrison, S. C.; Marinescu, D. C.; Nibert, M. L.; Baker, T. S. (2005). Features of Reovirus Outer Capsid Protein m1 Revealed by Electron Cryomicroscopy and Image Reconstruction of the virion at 7.0-Å Resolution, Structure 13(10): 1545-1557.

10. Zhang, X.; Tang, J.; Walker, S. B.; O'Hara, D.; Nibert, M. L.; Duncan, R. and Baker, T. S. (2005). Structure of Avian Orthoreovirus Virion by Electron Cryomicroscopy and Image Reconstruction, Virology 343:25-35.

11. Zhang, X.; Walker, S. B.; Chipman, P. R., Nibert, M. L., Baker, T. S. (2003). Reovirus polymerase lambda 3 localized by cryo-electron microscopy of virions at a resolution of 7.6 Å, Nature Structural Biology 10(12): 1011-8. (F1000 recommended)

12. Zhang, X., Zhang, X., Zhou, Z.H. (2010) Low cost, high Performance hybrid GPU-CPU solution for atomic resolution CryoEM single-particle reconstruction. J.Struct. Biol., 172, 400-406.
    

13. Kim, J.; Zhang, X.; Centonze, V. E., Bowman, V. D., Noble, S., Baker, T. S., Nibert, M. L. (2002). The hydrophilic amino-terminal arm of reovirus core shell protein lambda1 is dispensable for particle assembly, J. Virol. 76(23): 12211-22.

14. Luongo, C. L., Zhang, X., Walker, S. B., Chen, Y., Broering, T. J., Farsetta, D. L., Bowman, V. D., Baker, T. S., Nibert, M. L. (2002). Loss of activities for mRNA synthesis accompanies loss of lambda2 spikes from reovirus cores: an effect of lambda2 on lambda1 shell structure, Virology 296(1): 24-38.

15. Kong, Y., Zhang, X., Baker, T.S. & Ma, J. A Structural-informatics Approach for Tracing β-Sheets: Building Pseudo-Cα Traces for β-Strands in Intermediate-resolution Density Maps. Journal of molecular biology 339, 117-130 (2004).

16. Chandran, K., Zhang X., N. H. Olson, S. B. Walker, J. D. Chappell, T. S. Dermody, T. S. Baker and M. L. Nibert (2001). "Complete in vitro assembly of the reovirus outer capsid produces highly infectious particles suitable for genetic studies of the receptor-binding protein." Journal of virology 75(11): 5335-5342.

17. Chen, J; Settembre, E; Aoki, S; Zhang, X; Bellamy, A; Dormitzer; P; Harrison, S; Grigorieff, N. (2009). Molecular interactions in rotavirus assembly and uncoating seen by high-resolution cryo-EM, PNAS 106(26): 10644-10648. (F1000 recommended)
    

18. Odegard A.; Chandran K.; Zhang X.; Parker J. S.; Baker T. S.; Nibert M. L. (2004). Putative autocleavage of outer capsid protein m1, allowing release of myristoylated peptide m1N during particle uncoating, is critical for cell entry by reovirus, J. Virology. 78(16):8732-45.

19. Yan, X., G. Cardone, Zhang X., Z. H. Zhou and T. S. Baker (2014). "Single particle analysis integrated with microscopy: A high-throughput approach for reconstructing icosahedral particles." Journal of structural biology 186(1): 8-18.

20. Mrazek, J., D. Toso, S. Ryazantsev, Zhang, X., Z. H. Zhou, B. C. Fernandez, V. A. Kickhoefer and L. H. Rome (2014). "Polyribosomes Are Molecular 3D Nanoprinters That Orchestrate the Assembly of Vault Particles." ACS nano 8(11):11552-9

21. Ji, Y., D. C. Marinescu, W. Zhang, Zhang X., X. Yan and T. S. Baker (2006). "A model-based parallel origin and orientation refinement algorithm for cryoTEM and its application to the study of virus structures." Journal of structural biology 154(1): 1-19.

22. Estrozi LF, Settembre EC, Goret G, McClain B, Zhang X, Chen JZ, Grigorieff N, Harrison SC. (2013) Location of the dsRNA-dependent polymerase, VP1, in rotavirus particles. J Mol Biol. 425:124-132.
    

23. Bowman, V. D., Chase, E. S., Franz, A. W., Chipman, P. R., Zhang, X., Perry, K. L., Baker, T. S., Smith, T. J. (2002). An antibody to the putative aphid recognition site on cucumber mosaic virus recognizes pentons but not hexons, J. Virol. 76(23): 12250-8.